The upstream region from GeneX is placed upstream of CAT in a Reporter Plasmid. A TxF named PREB is known to affect GeneX transcription. PREB has 500aa and its gene is incorporated into a Producer Plasmid. Two other Producer Plasmids are also constructed: aa1-400 and aa100-500. Relative CAT activity is shown in the Table below for all three Producer Plasmids, as well as Reporter Plasmids with various regions deleted.. REPORTER PLASMID 5'-region of GeneX -1000-900-800-700-600 -500 -400-300 -200 -100 +1 ==== Region 1 2 3 4 5 =|==== 6 ==== ==== =|--CAT-- 7 8 9 10 THREE PRODUCER PLASMIDS REPORTER PLASMIDS 1-500aa aa100-500 aal-400 no deletion 100 250 0.4 delete [-1000 to -900] 100 250 0.4 delete [-1000 to 800] 250 250 1 delete [-1000 to 700] 250 250 1 delete [-1000 to 600] 250 250 1 delete [-1000 to 500] 250 250 1 delete [-1000 to 400] 1 1 1 delete [-1000 to 300] 1 1 1 delete [-1000 to 200] 1 1 1 delete [-1000 to 100] 1 1 1 11 <0.1 <0.1 <0.1 delete [-1000 to + Rationalize the results in the table. YOUR ANSWER MUST INCLUDE A TABLE LIKE THE ONE BELOW. 1. You MUST give the name of the most likely cis elements in each of the ten regions (1-10), as they relate to control of GeneX. Leave the table blank in any region where you do think there is NO cis element. At the bottom of both answers, you may comment on some of your conclusions. REQUIRED TABLE IN YOUR ANSWER Region cis element 1 2 3 4 5 6 7 8 9 10 1-100 150-350 400-500 2. Draw a picture of PREB that looks like the one to the right and label the most likely structural elements in each region. In the table also indicate where PREB binds. Hint: It will be easiest to answer this question if you start your thinking at the top of a deletion column and work down line-by-line (or start at the bottom and work up).