The wild-type ARK14 gene has two EcoRI cut sites, as shown in this diagram. A recessive disorder, known as ARK14 disease, is caused by an A-to-T point mutation in the bold underlined base in the ARK14 gene. ARK14 - GAATCC- 0 300 Part A GAATCC 700 900 An individual presenting with ARK14 disease came into a clinic. For the patient and immediate family members, PCR was performed on the ARK14 gene, followed by restriction digestion with EcoRI and RFLP and Southern blot analysis. 700 The results of the RFLP analysis are shown in this gel. (Note that thicker bands indicate that there are two bands at that location on the gel.) Use the information from the gel to create a pedigree for this family. 400 Complete this pedigree for the patient's family by dragging the symbols to their correct locations to indicate which family members have ARK14 disease and which do not. 300 200 I-1 I-2 II-1 II-2 Individual II-2 is pregnant; the father (II-3; not shown on the pedigree) does not have ARK14 disease. RFLP analysis was performed on II-3, and the doctor inform the couple that their child had no chance of having ARK14 disease. Us this information to deduce the results of the RFLP and Southern blot analysis for the father (II-3) on the gel below. Drag the bands to their correct locations on this gel showing the RFLP pattern for individual II-3. Use the label with a thick band to indicate two bands a position; use the blank label to indicate no band at a position. II-3 700 ? 600 400 300 200 ? ? ? ? reset?help