In E.coli, the expression from most operons relies on sigma factor 70 (070). A subset of operons relies on 032 which is expressed in place of σ70 in response to temp >42 °C (heat shock). Gene expressions from these latter operons is necessary for the bacteria to survive such a stressor. One or more of these genes also act through a positive feedback loop in order to maintain σ32 expression. RNA polymerase σ70 -35 -10 032 -35 10 typical genes heat shock genes To induce dsRNA expression against Gene X in your E.coli cultures, you grow them overnight in an incubator set to 37 °C in media that contains the appropriate nutrient signal. After you leave for the day, your lab mate who is growing a new thermophile adds his cultures to the same incubator and turns up the temperature to that bacteria's preferred 45 °C In the morning, you proceed with your experiment, feeding your E.coli cultures to your C. elegans and ultimately analyzing the resulting Gene X expression patterns using in situ hybridization. Q) Describe the mRNA expression pattern of Gene X (with respect to both location and quantity) you would expect to observe in your experimental population (C. elegans fed E.coli with the Gene X dsRNA containing operon) as compared to control worms. Please explain.