A. The sequence of hypothetical eukaryotic gene shown below. Only one strand of the double- stranded DNA is shown, this coding (or nontemplate) strand. Assume that the dashes represent more than one base-pair. The start of transcription is the A nucleotide closest to the arrow marked +1, transcription goes toward the right, and the splice junction sequences are underlined. Assume that transcription stops at the last base given. Mutant #2; A -TATAA----GATGCATCATAATGGATGCGTATAGTTAGGGCATTTTAACTTAA-3' Mutant #1:G ↑ 5'-CAAT--- What protein would be translated from the wild-type mRNA of this gene (no mutations)? B. The transcript of the above gene can be detected as a band of a certain size on a northern blot. Draw a diagram of a northern blot from a gel loaded with RNA extracted from the following three samples: wild- type RNA, mutant #1 (mutation of TATAA box to GATAA), and mutant #2 (mutation of the underlined G closest to the 5' end of the strand to an A). C. The following DNA sequence is part of an exon from the middle of a eukaryotic gene. Neither the start codon nor the stop codon are contained on this exon fragment. Only one strand of the double- stranded DNA is shown, this coding (or nontemplate) strand. Given that the C->T mutation shown results in a Leu-> Phe missense mutation, what protein sequence is encoded by this region of the wild-type gene (i.e. with C at the indicated position, not T)? Ignore any extile bases left over that do not form a complete codon. T 5'-TTCGGAGAGACCTTTCGCCC-3' a) ArgArgAspLeuSerPro b) PheGlyGluLeuPheArg c) SerGluArgProPheAla d) PheGlyGluThrPheArg e) GlyPhePheLeuArgAla