5. Portions of a large-scale preparation of a 10 kbp plasmid were digested with a number of restriction endonucleases. The results of these digests are summarized in the table below: Enzyme(s) BamHI EcoRI Pst I Not I Sst I Taq I BamHI-EcoRI BamHI-Pst I BamHI-Not I BamHI-Sst I BamHI-Taq I EcoR I-Sst I Pst I-Sst I Pst I-EcoRI Pst I-Taq I Not I-Taq I Not I-Sst I Not I-EcoR I Sst I-Taq I Fragment Sizes (kbp) 10.0 10.0 2.7, 7.3 10.0 4.8, 5.2 6.0, 10.0, 17.0, 23.0 4.7, 5.3 1.0, 1.7, 7.3 4.0, 6.0 2.5, 2.7, 4.8 10.0 2.0, 2.8, 5.2 1.0, 1.5, 2.7, 4.8 2.7, 3.0, 4.3 2.7, 7.3 10.0 1.5, 3.3, 5.2 1.3, 8.7 4.8, 5.2 (a) Construct a circular restriction map of the DNA molecule in question showing the relative location of all known restriction endonuclease sites. Draw this map to scale, where an arc of 36° = 1 kbp. (b) Identify and describe the nature of any apparently anomalous or unusual results in the table (above). Explain how these results can occur. (10 marks)