Q. 5. MCQ (Affinity chromatography) A. Affinity purification is based on: a. Charge b. Size c. Biorecognition d. Hydrophobicity B. If you hooked up adrenaline molecules to the sepharose beads, then you are most likely aiming at separating a. GPCR b. Insulin receptor c. Calmodulin receptor d. Glucose oxidase C. Which of the following malate dehydrogenase (MDH) has an 'epitope tag?? a. MDH-HA b. MDH-GST c. MDH-GFP d. All of them D. If you want to attach a tag to the C-terminus of your protein, you should add it a. Right after the STOP codon of the gene of interest b. Right before the STOP codon of the gene of interest c. Either way is OK d. None of them is OK. E. A diluted protein solution of 120kDa needs to be concentrated. SDS-PAGE analysis revealed that it has a couple more contaminating proteins with molecular weights 26, 75 kDa, which concentration filters would be the most appropriate to concentrate the given protein sample? a. 30kDa cut-off b. 50kDa cut-off c. 100kDa cut-off d. 150kDa cut-off