Problem 4 (non-porous) The values of KM and Vmax for an enzyme (at 21°C and pH = 7.1) are 0.004 kmol/m³ and 10 kmol/m³s, respectively. We immobilized this enzyme by attaching it covalently to the surface of acrylamide-based polymers (non-porous) that can be assumed to have spherical shape (diameter = 2 mm). The concentration of the substrate in the bulk and surface of acrylamide-based particles are 0.5 kmol/m³ and 0.0091 kmol/m³, respectively. The reaction takes place in a 1-Liter reactor where 1000 polymeric spheres with immobilized enzyme have been added. b) Estimate KL. Answer: 1.126 m/s