13. Graphical Analysis of Vmax max and Km The following experimental data were collected during a study of the cat- alytic activity of an intestinal peptidase with the substrate glycylglycine: Glycylglycine + H2O 2 glycine Product formed [S] (MM) (μmol/min) 1.5 0.21 2.0 0.24 3.0 0.28 4.0 0.33 8.0 0.40 16.0 0.45 Use graphical analysis (see Box 6-1 and its associated Living Graph) to determine the Km and Vmax for this enzyme prepa- ration and substrate.